These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of human VCAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
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